pdl1 deficient b16 Search Results


bsc40  (ATCC)
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b16/f10 cells  (GenScript corporation)
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b16-f10  (ATCC)
99
ATCC b16-f10
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crispr/cas9 system  (GenScript corporation)
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GenScript corporation crispr/cas9 system
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be0101  (Bio X Cell)
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Bio X Cell be0101
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2-(3-methoxybenzyl) succinic acid  (AstaTech Inc)
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AstaTech Inc 2-(3-methoxybenzyl) succinic acid
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colo 679 dsmz acc  (DSMZ)
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DSMZ colo 679 dsmz acc
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rvh 421 dsmz acc 127 human cell line  (DSMZ)
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DSMZ rvh 421 dsmz acc 127 human cell line
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mt3 boj  (DSMZ)
92
DSMZ mt3 boj
Figure 2. SLC13A3 diminishes tumor immunogenicity (A and B) Tumor growth curves (A), and representative tumor images (B) of Slc13a3+/+ and Slc13a3/ Yumm5.2 tumors in C57BL/6 mice (n = 4–5). Two-way ANOVA, ****p < 0.0001. (C and D) Tumor growth curves (C), and representative tumor images (D) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in C57BL/6 mice (n = 6–8). Two-way ANOVA, ****p < 0.0001. (E and F) Tumor growth curves (E) and tumor images (F) of Slc13a3+/+, Slc13a3/ <t>MT3</t> tumors in C57BL/6 mice (n = 10). Two-way ANOVA, ****p < 0.0001. (G and H) Percentages of IFNg+ CD8 T cells (G) and TNFa+ CD4 T cells (H) in parental or Slc13a3/ Yumm5.2 tumors, detected by FACS (n = 3). Two-tailed t test, *p < 0.05. (I and J) Tumor growth curves (I), and representative tumor images (J) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in NSG mice (n = 10–12). All data are presented as mean ± SEM. See also Figure S2.
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bp0090  (Bio X Cell)
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Bio X Cell bp0090
Figure 2. SLC13A3 diminishes tumor immunogenicity (A and B) Tumor growth curves (A), and representative tumor images (B) of Slc13a3+/+ and Slc13a3/ Yumm5.2 tumors in C57BL/6 mice (n = 4–5). Two-way ANOVA, ****p < 0.0001. (C and D) Tumor growth curves (C), and representative tumor images (D) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in C57BL/6 mice (n = 6–8). Two-way ANOVA, ****p < 0.0001. (E and F) Tumor growth curves (E) and tumor images (F) of Slc13a3+/+, Slc13a3/ <t>MT3</t> tumors in C57BL/6 mice (n = 10). Two-way ANOVA, ****p < 0.0001. (G and H) Percentages of IFNg+ CD8 T cells (G) and TNFa+ CD4 T cells (H) in parental or Slc13a3/ Yumm5.2 tumors, detected by FACS (n = 3). Two-tailed t test, *p < 0.05. (I and J) Tumor growth curves (I), and representative tumor images (J) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in NSG mice (n = 10–12). All data are presented as mean ± SEM. See also Figure S2.
Bp0090, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. SLC13A3 diminishes tumor immunogenicity (A and B) Tumor growth curves (A), and representative tumor images (B) of Slc13a3+/+ and Slc13a3/ Yumm5.2 tumors in C57BL/6 mice (n = 4–5). Two-way ANOVA, ****p < 0.0001. (C and D) Tumor growth curves (C), and representative tumor images (D) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in C57BL/6 mice (n = 6–8). Two-way ANOVA, ****p < 0.0001. (E and F) Tumor growth curves (E) and tumor images (F) of Slc13a3+/+, Slc13a3/ MT3 tumors in C57BL/6 mice (n = 10). Two-way ANOVA, ****p < 0.0001. (G and H) Percentages of IFNg+ CD8 T cells (G) and TNFa+ CD4 T cells (H) in parental or Slc13a3/ Yumm5.2 tumors, detected by FACS (n = 3). Two-tailed t test, *p < 0.05. (I and J) Tumor growth curves (I), and representative tumor images (J) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in NSG mice (n = 10–12). All data are presented as mean ± SEM. See also Figure S2.

Journal: Cancer cell

Article Title: Itaconate transporter SLC13A3 impairs tumor immunity via endowing ferroptosis resistance.

doi: 10.1016/j.ccell.2024.10.010

Figure Lengend Snippet: Figure 2. SLC13A3 diminishes tumor immunogenicity (A and B) Tumor growth curves (A), and representative tumor images (B) of Slc13a3+/+ and Slc13a3/ Yumm5.2 tumors in C57BL/6 mice (n = 4–5). Two-way ANOVA, ****p < 0.0001. (C and D) Tumor growth curves (C), and representative tumor images (D) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in C57BL/6 mice (n = 6–8). Two-way ANOVA, ****p < 0.0001. (E and F) Tumor growth curves (E) and tumor images (F) of Slc13a3+/+, Slc13a3/ MT3 tumors in C57BL/6 mice (n = 10). Two-way ANOVA, ****p < 0.0001. (G and H) Percentages of IFNg+ CD8 T cells (G) and TNFa+ CD4 T cells (H) in parental or Slc13a3/ Yumm5.2 tumors, detected by FACS (n = 3). Two-tailed t test, *p < 0.05. (I and J) Tumor growth curves (I), and representative tumor images (J) of Slc13a3+/+ and Slc13a3/ B16-F10 tumors in NSG mice (n = 10–12). All data are presented as mean ± SEM. See also Figure S2.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies V500 Rat Anti-Mouse CD45 (30-F11) BD Bioscience Cat# 561487; RRID: AB_10697046 FITC Rat Anti-Mouse CD90.2 (53–2.1) BD Bioscience Cat# 553003; RRID: AB_394542 Alexa Fluor 700 Rat Anti-Mouse CD8a (53–6.7) BD Bioscience Cat# 557959;RRID: AB_396959 APC-Cy 7 Rat Anti-Mouse CD4 (GK1.5) BD Bioscience Cat# 552051; RRID: AB_394331 BV786 Rat Anti-Mouse IFNg (XMG1.2) BD Bioscience Cat# 563773; RRID: AB_2738419 PE-Cy 7 Rat Anti-Mouse TNFa (MP6-XT22) BD Bioscience Cat# 557644; RRID: AB_396761 Rabbit Polyclonal Anti-SLC13A3 Thermo Fisher Scientific Cat# PA5-53224; RRID: AB_2647354 Monoclonal Anti-SLC13A3 The Zhiqiang An lab N/A xCT/SLC7A11 Antibody Cell Signaling Technology Cat# 98051; RRID: AB_2800296 Rabbit monoclonal Anti-NRF2 (D1Z9C) Cell Signaling Technology Cat# 12721; RRID: AB_2715528 Rabbit monoclonal Anti-PERK (C33E10) Cell Signaling Technology Cat# 3192; RRID: AB_2095847 Rabbit monoclonal Anti-GAPDH (D16H11) Cell Signaling Technology Cat# 5174; RRID: AB_11129865 Rabbit monoclonal Anti-b-ACTIN (D6A8) Cell Signaling Technology Cat# 8457; RRID: AB_10950489 InVivoPlus Anti-mouse PD-L1 (10F.9G2) Bio X Cell Cat# BE0101; RRID: AB_10949073 InVivoPlus rat IgG2b isotype control (LTF-2) Bio X Cell Cat# BP0090; RRID: AB_1107780 Chemicals, peptides, and recombinant proteins 2-(3-methoxybenzyl) succinic acid AstaTech Inc Cat# W13691 Liproxstatin-1 Cayman Chemical Cat# 17730 4-octyl Itaconate Cayman Chemical Cat# 25374 Itaconate Sigma Aldrich Cat# I29204 Critical commercial assays BODIPY 581/591 C11 Thermo Fisher Scientific Cat# D3861 BODIPY FL C12 Thermo Fisher Scientific Cat# D3822 BioTracker FerroOrange Live Cell Dye Sigma Aldrich Cat# SCT210-35NMOL 7-AAD Staining Solution BD Bioscience Cat# 559925; RRID: AB_2869266 Deposited data Raw Single cell RNA-seq This paper [GEO Database]: [GSE268205] Bulk RNA-seq raw data 1 This paper [GEO Database]: [ GSE268293] Experimental models: Cell lines Mouse cell line: B16-F0 ATCC CRL-6322 Mouse cell line: B16-F10 ATCC CRL-6475 Mouse cell line: Yumm5.2 ATCC CRL-3367 Mouse cell line: MC38 Tanikawa et al.,51 N/A Mouse cell line: MT3 Boj et al.,52 N/A Human cell line: OVCAR3 ATCC HTB-161 Human cell line: A375 ATCC CRL-1619 Human cell line: COLO-679 DSMZ ACC-264 Human cell line: RVH-421 DSMZ ACC-127 Human cell line: OC8 Cui et al.,50 N/A Experimental models: Organisms/strains Mouse: NOD.SCID gc deficient (NSG) The Jackson Laboratory IMSR_JAX:005557 Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 (Continued on next page) e1 Cancer Cell 42, 1–13.e1–e6, December 9, 2024

Techniques: Immunopeptidomics, Two Tailed Test